(eBook PDF) Fundamental Laboratory Approaches for Biochemistry and Biotechnology 2nd Edition – Digital Ebook – Instant Delivery Download

Product details:

• ISBN-10 ‏ : ‎ 0470087668
• ISBN-13 ‏ : ‎ 978-0470087664
• Author: Alexander J. Ninfa

Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills, allowing students to learn techniques and develop the the critical thinking and organizational approaches necessary to conduct laboratory research.

Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques but also includes molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques.  The experiments are designed so that theory and technique are learned as fundamental research tools, and the biochemistry and molecular biology applications are seamlessly integrated throughout the manual. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals.  Most importantly, perhaps, is the authors’ three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual’s experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols.   In this way, students and instructors are able to break away from a “cookbook” approach and to think and investigate for themselves.

Table contents:

Chapter 1: Getting Started in Scientific Research

Chapter 2: Basic Procedures in the Biochemistry Laboratory

Chapter 3: Spectroscopic Methods

Chapter 4: Quantification of Protein Concentration

Chapter 5: Chromatography

Chapter 6: Gel Electrophoresis of Proteins

Chapter 7: Overview of Protein Purification

Chapter 8: Subcellular Fractionation

Chapter 9: Isolation and Characterization of an Enzyme-Alkaline Phosphatase from Escherichia Coli

Chapter 10: Enzyme Kinetics

Chapter 11: Ligand Binding

Chapter 12: Enzymatic Methods of Analysis

Chapter 13: Recombinant DNA Techniques

Chapter 14: Polymerase Chain Reaction (PCR) Technology

Chapter 15: Using the Computer and the Internet for Biochemical Research and Communication

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